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Clicking on the donut icon will load a page at altmetric. Find more information on the Altmetric Attention Score and how the score is calculated. Many different methods Molndal grier dating used for mass-spectrometry-based protein quantification in pharmacokinetics and systems pharmacology. It grire not been Massage parlour in Umea Sweeden to what extent the results from these various methods are comparable.
Here, we compared six different mass spectrometry-based proteomics methods by measuring the expression of clinically relevant drug transporters and metabolizing enzymes in human liver. Mean protein concentrations were in general Molndal grier dating to similar levels by methods using whole tissue lysates.
Methods using subcellular membrane fractionation gave incomplete enrichment of the proteins. When the enriched proteins were adjusted to levels in whole tissue lysates, they were on average 4-fold lower than those quantified directly in whole tissue lysates. Molndal grier dating
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The differences in Molndal grier dating levels were propagated into differences in predictions of hepatic clearance. In conclusion, caution is needed when comparing and applying quantitative proteomics data obtained with different methods, especially since membrane fractionation is common practice for protein quantification used in drug clearance predictions.
Detailed description of the proteomic methods, supporting figures and tables PDF. Protein concentrations of all quantified proteins in the ten liver samples obtained in the different laboratories XLSX.
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